Raw Cytokine Data

• Raw Cytokine Data 2009
• Raw Cytokine Data 2011
• Raw Cytokine Data 2013

200FG

Cytokine QTL Data

• CA IL10

• CA IL1Ra

• CA IL1β

• CA IL6

• CA IL8

• CA TNFα

• LPS IL10

• LPS IL1Ra

• LPS IL1β

• LPS IL6

• LPS IL8

• LPS TNFα

• MTB IL10

• MTB IL1Ra

• MTB IL6

• MTB IL8

• MTB IL1β

500FG

Client Records

• Private Records

• Public Records

Questionnaire on demographic data, health, gastro-intestinal condition, diet, exercise and smoking.

500FG

Cytokines

Several bacterial, fungal and viral stimuli were used in ex-vivo experiments. IFN-y, IL-1b, IL-6 and TNF-a production were measured in whole blood experiments. IFN-y, IL-1b, IL-6, IL-17, IL-22 and TNF-a production were measured in supernatants of PBMC experiments. IL-6 and TNF-a production were measured in supernatants of serum-derived macrophage experiments.

• 489 samples

500FG

Cytokine QTL data (available as zip file)

500FG

Thyroid hormones

Concentration of TSH (mE/l) and fT4 (pmol/l) measured in heparin plasma.

• 488 samples

500FG

Cell staining

cell counts per sample

• 516 samples

500FG

cell staining counts QTL (available as zip file)

500FG

Serum Ig levels

Ig levels per sample

• 489 samples

500FG

Metabolome

Using plasma derived from EDTA blood tubes we measured a whole range of circulating metabolites mainly involving amino acid, glucose and lipid metabolism. In total, there are 231, 1589 and 8614 metabolic features measured by three platforms (1) (2) (3), respectively.

(1) A nuclear magnetic resonance (NMR) approach (BM, Brainshake Metabolomics/ Nightingale Health platform, Finland)

(2) flow-injection TOF-M (GM, General Metabolomics, Boston)

(3) an integrated measurement system of NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) (UM, untargeted metabolomics, U.S.A)

• 458 samples (General Metabolomics, Boston)

• 391 samples (Nightingale Health/Brainshake platform, Finland)

• 531 samples (untargeted metabolomics, USA)

500FG

Metabolite QTL data (available as tar.gz file)

500FG

Platelet activation

Platelet activation was measured on 8 consecutive time points using FACS after stimulation with ADP or Collagen in combination with Pselectin or Fibrinogen.

• 534 samples

500FG

Circulating plasma factors

Different systemic markers measured at baseline (without stimulation) in either EDTA plasma or serum. 

• 489 samples

500FG

Uric acid

Uric acid levels (mmol/L) were measured systemically at baseline (i.e. without stimulation) in heparin plasma.

• 309 samples

500FG

Microbiome

Link to data

As part of the 500 Functional Genomics (500FG) project, stool samples from 471 healthy adults were subjected to metagenomic shotgun sequencing (one time point per individual). Concurrently, blood samples were collected from each individual and the cytokine production was measured in response to bacterial and fungal stimulations. Significant inter-individual variation was observed in the gut microbial profiles as well as the immunological profiles. Subsequent association testing showed that microbiome-host interactions modulate the inflammatory cytokine production capacity in healthy individuals.

500FG

Flow cytometry data

Raw flow cytometry data files measured using 4 different 10-color antibody panels (General, T cell, B cell and intracelluar Tcell/Treg panel).

300OB

Link to data

Data from the paper "Sex-Specific Regulation of Inflammation and Metabolic Syndrome in Obesity", by ter Horst et al. (https://doi.org/10.1161/ATVBAHA.120.314508; Arteriosclerosis, Thrombosis, and Vascular Biology. 2020;40:1787-1800)